Matrix-assisted laser desorption ionization-time of flight mass spectrometry-based single nucleotide polymorphism genotyping assay using iPLEX gold technology for identification of Mycobacterium tuberculosis complex species and lineages.

MALDI - TOF MS - based SNP genotyping assay using the iPLEX Gold technology 1 for identification of Mycobacterium tuberculosis complex species and lineages

DNA preparation for Sequenom MALDI-TOF based human SNP genotyping using the prepGEMTM Storage card blood kit

Completed sequences of the human genome have provided biomedical researchers with opportunities to investigate genetic diversity in academic, clinical and commercial areas. Single nucleotide polymorphisms (SNPs) are generally accepted as the most important markers owing to their abundance and simplicity; thus rendering straightforward assay design and scoring (1). They are useful in large studi...

A Microfluidic Device for Detection of Single Nucleotide Polymorphisms by Allele Specific Single Base Extension

We present a microfluidic device with integrated temperature sensors and heaters for single nucleotide polymorphism (SNP) detection. The allele specific primer is immediately annealed next to the polymorphic site, and extended by a single base. The extension product is then purified by solid phase capture, and subsequently released via chemical cleavage. After desalting, the released product is...

Facile method for automated genotyping of single nucleotide polymorphisms by mass spectrometry.

In the future, analysis of single nucleotide polymorphisms (SNPs) should become a powerful tool for many genetic applications in areas such as association studies, pharmacogenetics and traceability in the agro-alimentary sector. A number of technologies have been developed for high-throughput genotyping of SNPs. Here we present the simplified GOOD assay for SNP genotyping by matrix-assisted las...

Evaluation of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry in Identification of Nontuberculous Mycobacteria.

BACKGROUND/AIMS Species-level identification of nontuberculous mycobacteria (NTM) is important in making decisions about the necessity and choice of antimicrobial treatment. The reason is predictable clinical significance and the susceptibility profile of specific NTM species. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is recognized as a diagnost...